2024 Hct116 cells细胞

Hct116 cells细胞

Author: tiym

August undefined, 2024

Web人结直肠腺癌细胞HCT116细胞是1979年BrattainM等从患结肠癌的男性病人中分离建立的3株恶性细胞之一；产生CEA、角蛋白。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。病理三级。 http://www.xpbiomed.com/productinfo/1396153.html?templateId=1133605#:~:text=HCT116%20is%20a%20line%20of%20human%20colorectal%20carcinoma,HCT116%20cells%20as%20a%20model%20for%20colorectal%20cancer.

警惕！细胞系身份有误致多篇重量级论文撤稿！ - 知乎

WebHCT116 and HT29 cells demonstrate high cancer stemness due to the overexpression of CD133 and CD44 [5]; by contrast, CD133 is absent in hepatocellular carcinoma cells [6]. Furthermore, regorafenib ... WebHCT116 cells were transfected with 10 nM control siRNA (siCtrl) or siRNA targeting EI24 or ATG5. (A) The mRNA levels of EI24, ATG5, and GAPDH were analyzed by reverse-transcription PCR. (B) The protein levels of EI24 ATG5 and β-actin were analyzed by western blotting. Representative data are shown. defined threshold inside classes is set to 11

5-FU promotes stemness of colorectal cancer via p53-mediated …

WebFeb 5, 2024 · hct116呈上皮细胞样，多角状，贴壁生长。核型接近二倍体，62% 的细胞染色体数为45，多倍体出现率为6.8% 。该细胞是个合适的转染宿主，常用于高通量筛选、 … WebCD133 is not an appropriate CSC marker for HCT116 cells. We examined several cell lines for the presence of subpopulations of cells that expressed CD133, which is a generally accepted CSC marker in primary colon cancer.(16,17) Consistent with a previous report,(18) >70% of WiDr and HT29 colon cancer cells were positive for CD133 (CD133 WebIn addition, CD133+CD44+ HCT116 cells were subcutaneously injected in xenograft mice and analyzed the effect of BC on tumor formation, tumor volume, and CSCs markers in tumors. Results: BC ... feeling first movements in pregnancy

入侵脑细胞.The Cell (2000) 1080p BluRay.mp4_BT/迅雷下载-片吧网

人结直肠腺癌细胞HCT116-上海细胞库

WebNov 24, 2024 · Purpose Cisplatin resistance is still a serious problem in the clinic. However, the underlying mechanism remains unknown. In our study, we investigated cisplatin resistance by using the cisplatin-resistant cell line HCT116R. Methods The HCT116 cell line, a colon cancer cell line, was purchased. Cell viability was determined … WebIt has been shown that the exposure to 150–200 μM of [6]-gingerol of a colorectal cell line (HCT-116) reduce by 22% and 28% cell growth rate, respectively, after the 72 h incubation. And, it is also observed in other human colorectal cancer cell lines including SW480, HT-29, LoVo, and Caco-2 cells treated with 200 μM of [6]-gingerol for 72 ... defined the scopeWebOct 21, 2024 · b Confocal images of immunofluorescence staining of HCT116 cells treated with or without 5-FU and the mean intensity of β-catenin in p53 low and p53 high cells. Scale bar = 20 μm. Scale bar = 20 ... defined throat human design

"Web6 hours ago · 两篇Cell：揭示中性粒细胞在肿瘤免疫疗法中的重要作用，或为免疫疗法成败的关键！. 肿瘤能否被治愈，一直是医学界专注研究的问题。. 近年来 ... " - Hct116 cells细胞

Hct116 cells细胞

Isolation of CD133 + CD44 + HT29 and CD133 + CD44

WebHCT 116 BRCA2 KO cells were created through targeted disruption of human BRCA2 by homologous recombination and deletion was confirmed by Southern Blot and Western … HCT116 cells have a mutation in codon 13 of the KRAS proto-oncogene, and are suitable transfection targets for gene therapy research. The cells have an epithelial morphology and can metastasize in xenograft models. When transducted with viral vectors carrying the p53 gene, HCT116 cells remain arrested in … See more HCT116 is a human colon cancer cell line used in therapeutic research and drug screenings. See more HCT116 cells are used in a variety of biomedical studies involving colon cancer proliferation and corresponding inhibitors. The cell line has … See more • Cellosaurus entry for HCT116 See more

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WebFeb 5, 2024 · HCT116，人结肠癌细胞，是1979年M.Brattain等从患结肠癌的男性病人中分离的三株恶性细胞之一，在半固体琼脂糖培养基中形成克隆。 HCT116在无胸腺的裸鼠有致瘤性，可形成上皮样的肿瘤结块和转移灶。携带原癌基因第1… Web选取人结肠癌细胞株HCT116由新疆医科大学附属肿瘤医院实验室提供，miR-30a慢病毒表达载体GV369委托上海吉凯基因公司设计合成，Luc荧光素酶质粒购自上海吉凯基因化学技术有限公司(编号GV306)，转染试剂由吉凯公司内部配制。

WebCell Line Name HCT 116 (Human Colorectal Carcinoma Cells) Synonyms HCT-116; HCT.116; HCT_116; HCT116; CoCL2 Organism Homo sapiens (Human) Tissue Colon Cell Type － Morphology Epithelial Disease Colorectal carcinoma ... 细胞计数：一般要求冻存的细胞，每毫升的细胞数量为1×10 6-10 7 个细胞。 Web细胞描述： HCT116是1979年M.Brattain等从患结肠癌的男性病人中分离的三株恶性细胞之一。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。细胞特性： 1）来源：人类，男性， …

WebApr 9, 2024 · 这是因为，Cellosaurus数据库已认定HepG2是错误识别的细胞株(Misclassified Cell Line)。有文献(PubMed=19751877)报道，HepG2实质为肝母细胞瘤细胞株而非肝细胞性肝癌细胞株。 ... 期刊主编发现实验中使用的HCT116和SW480两株细胞被hela污染，文章中数据不具有可信性。 ... WebWe have used a lentiviral vector to stably express p53 at a physiological level in p53 knockout HCT116 cells. Cells transduced with wild type p53 responded to genotoxic stress by stabilizing p53 and expressing p53 …

WebLenti-AP1-TA-Luc-EF1α-mCherry (10^8TU/ml, 报告基因慢病毒)，即Lenti-AP1-TA-Luc-EF1α-mCherry-T2A-Puro，是碧云天自行研发的一种可以在大多数哺乳动物细胞(包括原代细胞和干细胞)中表达AP1报告基因并组成性表达mCherry和嘌呤霉素抗性基因的重组慢病毒。

Web• ™HCT116-Dual cells are guaranteed mycoplasma-free. CELL LINE DESCRIPTION HCT116-Dual™ cells are adherent epithelial cells that have been stable integration of two inducible reporter constructs. HCT116 cells are commonly used to study inflammatory responses in colon epithelial cells. HCT116-Dual™ cells express a secreted embryonic defined thatWebHCT 116细胞是由M·Brattain等人于1979年从患结肠癌的男性病人中分离的三株恶性细胞中的一株。HCT 116细胞在半固体琼脂糖培养基中形成克 … defined throat centerWebColorectal cancer cells Caco-2 and HCT116 resist epigenetic effects of isothiocyanates and selenium in vitro. These data suggest that treatment for colon cancer cells with selenium … feeling fishingWebNov 27, 2024 · 综上所述，miR-31可促进结肠癌细胞HCT116细胞焦亡通路中NLRP3、Caspase-1、GSDMD-N、IL-18、IL-1β蛋白的表达，其靶基因SATB2具有同样的作用，提示miR-31靶向SATB2上调细胞焦亡通路中的蛋白表达，促进结肠炎相关肠癌的发生发展，为肠炎和肠炎相关肠癌的发生机制提供了 ... defined territory of taiwanWebNov 27, 2024 · 综上所述，miR-31可促进结肠癌细胞HCT116细胞焦亡通路中NLRP3、Caspase-1、GSDMD-N、IL-18、IL-1β蛋白的表达，其靶基因SATB2具有同样的作用，提 … defined throatWebHCT116 is a human colorectal carcinoma cell line initiated from an adult male. The cells are adherent with an epithelial morphology. Following implantation into immunocompromised … define dtm ap human geographyWeb细胞划痕实验及Transwell侵袭实验结果表明miR-455-3p mimics能显著降低HCT116细胞迁移及侵袭能力，此结果与miR-455-3p mimics在三阴性乳腺癌细胞MDA-MB-231及MDA-MB-468中的作用一致 [ 9] ，说明过表达miR-455-3p能显著抑制HCT116细胞迁移及侵袭。. MMPs能够通过降解细胞外基质成分 ... defined thus