2024 Mitomycin c prophage

Mitomycin c prophage

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August undefined, 2024

Web6 dec. 2024 · Mitomycin C acts on DNA in three possible ways: promoting (a) cross-linking events, (b) alkylation and not necessarily cross-linking, and (c) strand breakage. … WebTo understand the function of these prophages, cultures of TM1040 were treated with mitomycin C to induce the production of viral particles. A significant increase in viral counts and a decrease in bacterial counts when treated with mitomycin C suggested that prophages were induced from TM1040.

Mitomycin C 50-07-7 - Sigma-Aldrich

WebProphage Induction Causes Geobacter Electroactive Biofilm Decay. Liu, Ye, Zhang, Rensing, Zhou, Nealson ... and a mitomycin C induction assay revealed the lysogenic to lytic transition of those prophages, resulting in a progressive decay in both current generation and the EAB. WebChemical treatment of lysogenic strains with Mitomycin C is known to cause induction of temperate phages, though it is not always easy to clearly identify a lysogenic strain or to … cleveland co sheriff oklahoma

How to propagate & concentrate the prophage after induction …

Web10 jan. 2024 · Using viral and bacterial mutants in our EHEC disease mouse model, we showed that whereas an inducing signal needed to begin viral replication was essential for lethal disease, virus production was not: … Web1 dec. 1995 · A prophage was induced from cells of the pathogenic spirochaete Serpulina hyodysenteriae using mitomycin C. Five to seven hours after mitomycin C was added … blythe chevrolet

A Metabolite of Pseudomonas Triggers Prophage-Selective …

Prophage Induction Causes Geobacter Electroactive Biofilm Decay.

WebMitomycin C has also been used topically rather than intravenously in several areas. The first is cancers, particularly bladder cancers and intraperitoneal tumours. It is now well … WebMitomycin C has also been used topically rather than intravenously in several areas. The first is cancers, particularly bladder cancers and intraperitoneal tumours. It is now well known that a single instillation of this agent within 6 hours of bladder tumor resection can prevent recurrence. blythe chp officeWebExposure to mitomycin C induces no DNA breakdown in these mutants (Boyce & Howard-Flanders, 1964). A recA uvr A double mutant was first constructed by Howard-Flander & Boycse (1966). The ultraviolet dose for 37 % survival of the double mutant is 0-2 erg/mm2, corresponding approximately to one dimer per bacterial genome (Howard-Flanders, 1968). cleveland cosmetic surgery lakewood ohio

"WebTranscript levels of prophage-encoded genes in strain MGAS315 during culture with mitomycin C, hydrogen peroxide, and D562 epithelial cells relative to transcript levels under control conditions. Gene names are listed on the y axis. " - Mitomycin c prophage

Mitomycin c prophage

Induction of phage formation in the lysogenic Escherichia coli K …

Web19 nov. 2014 · In response to mitomycin C induction, virus-like particles can increase to 7 × 109 per ml, while host cells decrease an order of magnitude. The vB_CibM-P1 … Web21 dec. 2024 · In contrast, in the presence of mitomycin C, the fitness benefits of ARGs relative to prophages were less pronounced or even absent, and lysogen fitness was primarily determined by prophage carriage (in Cluster 1 there was a higher benefit of prophages than ARGs, and mitomycin C was present in most of these treatment …

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Web17 jun. 2024 · Previous data suggest that gene exchange may occur in Yersinia. Only scarce information exists about temperate phages of Y. enterocolitica, even though many prophage sequences are present in this species. We have examined 102 pathogenic Y. enterocolitica strains for the presence of inducible prophages by mitomycin C treatment. WebThe mitomycins are antitumor antibiotics that are under investigation now for more than 30 years. Mitomycin C (MMC) is the best investigated subtype. It serves as a prototype for drugs with bioreductive alkylation, which is a unique feature of this class. MMC is mainly active under anaerobic circumstances.

WebMitomycin C-treated bacteria resumed DNA synthesis also when infected with either T 3 or T 5 phage. 3. 3. Although mitomycin C did not affect the infectivity of free phage, the reproduction of phage was progressively impaired with increasing concentrations of the antibiotic. Phage-infected bacteria in contact with high levels of mitomycin C ... WebMitomycin C and UV-C are commonly used to in- duce lysogens (Ackermann & DuBow 1987). Based on studies in the phage lambda (Gottesman & Oppenheim 1994), …

WebInduction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. Induction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C. … WebMitomycin C (215a) shows a potent activity against a wide spectrum of tumors and bacteria. In spite of its high toxicity it is extensively used in chemotherapy especially in treating gastrointestinal cancers 〈B-80MI 801-01〉. For synthetic chemists the structure of mitomycins presents a formidable challenge.

Web16 jun. 2024 · Gene Map and Features of Φstx 2dact prophage. Lambdoid phage Φ1720a-02 was originally isolated from EC1720a-02, a STEC strain found in packaged ground beef [].To create the novel C. rodentium-mediated mouse model of EHEC infection, C. rodentium DBS100 (also known as C. rodentium strain ICC 168 (GenBank accession number …

WebInduction of phage formation in the lysogenic Escherichia coli K-12 by mitomycin C Nature. 1959 Oct 3;184(Suppl 14):1079-80. doi: 10.1038/1841079b0. Authors N OTSUJI, M SEKIGUCHI, T IIJIMA, Y TAKAGI. PMID: 14429381 DOI: 10.1038/1841079b0 No abstract available. MeSH terms ... cleveland co soWebIn the laboratory, the most common induction method is the DNA damaging chemical Mitomycin C. Although pH has been listed in the literature as an induction method, it is not widely used. Here, we detail a protocol for prophage induction by … blythe chorn rambollWeb19 jul. 2024 · Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis Detecting Lactococcus lactis Prophages by Mitomycin C-Mediated Induction Coupled to Flow Cytometry Analysis Front Microbiol. 2024 Jul 19;8:1343. doi: 10.3389/fmicb.2024.01343. eCollection 2024. Authors cleveland cosmetology schoolWeb15 feb. 2024 · Our results showed that mitomycin C exposure induced an up-regulation of and lexA suggesting activation of SOS response. In addition, mitomycin C and UV exposure seems to lead to up-regulation of genes in … blythe chp scaleWeb新井浩 is an academic researcher. The author has contributed to research in topic(s): Lactobacillus salivarius. The author has an hindex of 2, co-authored 3 publication(s) receiving 20 citation(s). cleveland co tax collectorWebMitomycin C (MMC) acts as a prototype for drugs with bioreductive alkylation. It is mainly found to be active under anaerobic conditions. MMC exhibits a vast clinical … cleveland cosmetic surgery lakewoodWebNational Center for Biotechnology Information cleveland cosmetic surgery