WebbTransposase (Tnp) Tn5 is a member of the RNase superfamily of proteins which includes retroviral integrases. Tn5 can be found in Shewanella and Escherichia bacteria. The … Webb1 dec. 2014 · This work presents simple and robust procedures for Tn5 transposase production and optimized reaction conditions for tagmentation-based sequencing library …
CN109680343B - Library building method for exosome micro DNA
Webb22 juli 2024 · Utilizing highly saturated Tn5 libraries and more than a quarter-billion reads from Tn5-genomic junctions, we identified the conditionally essential genes in S. Typhimurium that are required for the growth under varying levels of iron restriction. WebbTransposon DNA sequence + EZ-Tn5™ Transposase enzyme Transposomics provides a fast and easy method for generating a library of DNA sequences with insertional mutations, or a library of live mutant bacteria. Many genomics applications bene˜ t from the high e˚ ciency and low bias of the EZ-Tn5 system, including metagenomics, strain develop- coffee associates nj
novel strand-specific RNA-sequencing protocol using dU-adaptor ...
WebbA library construction method of exosome micro-DNA, comprising: embedding a DNA sequence comprising a transposon end sequence and a tag sequence with a TN5 enzyme into a TN5 enzyme complex; incubating exosomes, the TN5 enzyme complex and reaction buffer containing IGEPAL together, lysing the exosomes and fragmenting exosome DNA; … Webb24 maj 2024 · Initially the libraries were prepared using the Tn5 transposase (Fig. 1 a), or with the process of DNA fragmentation, end-polishing, adaptors ligation (Fig. 1 b), after PCR amplification and purification, in contrast with the traditional protocol where size selection and quantification occurred using the one sample one tube method (Fig. 1 c), … http://accuquantbio.life.tsinghua.edu.cn/images/publications/2024_Chen_ACS_Appl._Mater._Interfaces.pdf calypso agency